Coding

Part:BBa_K1772001:Design

Designed by: Tianyu Tan   Group: iGEM15_Purdue   (2015-09-17)


Manganese Peroxidase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 50
    Illegal PstI site found at 722
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 50
    Illegal PstI site found at 722
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 492
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 50
    Illegal PstI site found at 722
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 50
    Illegal PstI site found at 722
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This gene sequence excludes introns (noncoding segments) present in the original gene from Heterobasidion irregulare TC 32-1. Sequence optimization was done using IDT's codon optimizer. Illegal restriction enzyme sites (under RFC 10 definition) were detected using NEBcutter2 and removed with silent mutations.


Source

Heterobasidion irregulare TC 32-1

References